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We also post guided exercises as part of our educational outreach effort. Simple nuclei identification tutorial ( sample data) (courtesy of the German BioImaging network) Performing a colocalization assay ( relevant example pipeline) Using the Worm Toolbox for image analysis of C. Identifying and measuring cells: Cytoplasm-nucleus translocation assay ( relevant example pipeline)Ĭalculating and applying illumination correction for images ( relevant example pipeline) Identifying, measuring, and classifying yeast colonies ( relevant example pipeline) Using the Input modules in CellProfiler 2.1: ![]() Using CellProfiler for Quantitative Image Analysis The NIH has published a introductory chapter of “best practices” for image-based high-content screening (in which CellProfiler is mentioned) as part of the Assay Guidance Manual, and our group has published a more advanced follow-up chapter on image analysis methods. More sample images and information on the image data can be found on the Broad Bioimage Benchmark Collection (BBBC). Our introduction to automated image analysis principles and practicalities is published as an educational article at PLoS. CellProfiler Analyst Example Data and Properties File Example database, configuration file, and images: Single 96-well plate of human cellular images, for use with CellProfiler Analyst 2.0. Technical descriptions of CellProfiler and CellProfiler Analyst software can be found in our papers while more written tutorials can be found on the CellProfiler GitHub page. ![]() Visit our YouTube playlist for video tutorials on CellProfiler, CellProfiler Analyst, segmentation strategies, how to construct pipelines, and much more.
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